Effects of sublethal fipronil exposure on cross-generational functional responses and gene expression in Binodoxys communis.

The effective systemic insecticide fipronil is widely used on a variety of crops and in public spaces to control insect pests. Binodoxys communis (Gahan) (Hymenoptera: Braconidae) is the dominant natural enemy of Aphis gossypii Glover (Homoptera: Aphididae), an important cotton pest, and has good efficiency in inhibiting aphid populations. The direct effects of environmental residues of sublethal fipronil doses on adult B. communis have not previously been reported. This study therefore aimed to evaluate the side effects and transcriptomic impacts of sublethal fipronil doses on B. communis. The results showed that exposure to the LC10 dose of fipronil significantly reduced the survival rate and parasitism rate of the F0 generation, but did not affect these indicators in the F1 generation. The LC25 dose did not affect the survival or parasitic rates of the F0 generation, but did significantly reduce the survival rate of F1 generation parasitoids. These results indicated that sublethal doses of fipronil affected B. communis population growth. Transcriptome analysis showed that differentially expressed genes (DEGs) in B. communis at 1 h after treatment were primarily enriched in pathways associated with fatty acid elongation, biosynthesis of fatty acids, and fatty acid metabolism. DEGs at 3 days after treatment were mainly enriched in ribosomal functions, glycolysis/gluconeogenesis, and tyrosine metabolism. Six DEGs (PY, ELOVL, VLCOAR, MRJP1, ELOVL AAEL008004-like, and RPL13) were selected for validation with real-time fluorescent quantitative PCR. This is the first report of sublethal, trans-generational, and transcriptomic side effects of fipronil on the dominant parasitoid of A. gossypii. The results of this study show that adaptation of parasitoids to high concentrations of pesticides may be at the expense of their offspring. These findings broaden our overall understanding of the intergenerational adjustments used by insects to respond to pesticide stress and call for risk assessments of the long-term impacts and intergenerational effects of other pesticides.

Assessment of the risk of imidaclothiz to the dominant aphid parasitoid Binodoxys communis (Hymenoptera: Braconidae).

The neonicotinoid of imidaclothiz insecticide with low resistance and high efficiency, has great potential for application in pest control in specifically cotton field. In this systematically evaluate the effects of sublethal doses of imidaclothiz (LC10: 11.48 mg/L; LC30: 28.03 mg/L) on the biology, transcriptome, and microbiome of Binodoxys communis, the predominant primary parasitic natural enemy of aphids. The findings indicated that imidaclothiz has significant deleterious effects on the survival rate, parasitic rate, and survival time of B. communis. Additionally, there was a marked reduction in the survival rate and survival time of the F1 generation, that is, the negative effect of imidaclothiz on B. communis was continuous and trans-generational. Transcriptome analysis revealed that imidaclothiz treatment elicited alterations in the expression of genes associated with energy and detoxification metabolism. In addition, 16S rRNA analysis revealed a significant increase in the relative abundance of Rhodococcus and Pantoea, which are associated with detoxification metabolism, due to imidaclothiz exposure. These findings provide evidence that B. communis may regulate gene expression in conjunction with symbiotic bacteria to enhance adaptation to imidaclothiz. Finally, this study precise evaluation of imidaclothiz's potential risk to B. communis and provides crucial theoretical support for increasing the assessment of imidaclothiz in integrated pest management.

Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae).

Introduction: Bacteria play critical roles in the reproduction, metabolism, physiology, and detoxification of their insect hosts. The ladybird beetle (Harmonia axyridis) harbors a myriad of endosymbiotic microbes. However, to date, little is known about how the microbial composition of H. axyridis varies throughout its life cycle. Methods: In this study, 16S rRNA amplicon sequencing and quantitative PCR were employed to investigate the diversity and dynamics of bacterial symbionts across the egg, larval, pupae, and adults stages of H. axyridis. Results: Higher bacterial community richness and diversity were observed in eggs, followed by those in adults and pupae. The community richness index differed significantly between second-instar larvae and other developmental stages. Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla. Staphylococcus, Enterobacter, Glutamicibacter, and Acinetobacter were the dominant bacteria genera; however, their relative abundances fluctuated across host developmental stages. Interestingly, the larval stage harbored high proportions of Firmicutes, whereas the adult microbial community largely consisted of Proteobacteria. Discussion: This study is the first to determine the symbiotic bacterial composition across key life stages of H. axyridis. These outcomes can foster the development of environmental risk assessments and novel biological control strategies.